An in vitro assessment of the responses of human dermal fibroblast seeded on 3D printed thermoplastic polyurethane scaffold

Tissue engineering is a multidisciplinary field an interdisciplinary for the design of biological substitutes that can improve, restore, and maintain tissue functions. Thermoplastic polyurethanes (TPUs) are linear polymers which widely used due to its flexibility in processing methods, biocompatibility excellent mechanical properties. They suitable materials use three-dimensional (3D) printing. Dermal fibroblasts mesenchymal cells play crucial roles physiological repair. The present study aimed investigate viability, proliferation, adhesion, type IV collagen expression human dermal (HDFs) seeded on 3D printed TPU scaffolds vitro. HDFs were or culture polystyrene plates as control cultured 1, 3, 7, 14 days. prepared using custom made fused deposition modelling printer. viability proliferation was analyzed by WST-1 assay days 1 3. cell adhesion evaluated scanning electron microscopy (SEM) morphology examined hematoxylin eosin (H&E) staining. Expression immunohistochemical (IHC) lower than their groups slightly higher day SEM images showed HDF attachment scaffold surface with spindle-shaped morphology. H&E staining demonstrated smaller morphologies 7 compared Type more intense 14. In conclusion, our shows potential applications skin fibroblasts.